Plastination
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What
is plastination?
A
method of preserving perishable biological specimens by replacing
the tissue water and lipid with a curable plastic polymer. The
specimens preserved in this manner are permanent, clean, non-toxic
and dry.
- How
is it done? - There are a variety of different plastination
methods available. Each has advantages and disavantages and yeilds
specimens with different characteristics.
- Why
is it useful in Anatomy? - Plastinated specimens retain
textures and structures of tissue and are therefore an invaluable
teaching resource in anatomy. Plastinated specimens have none
of the usual hazards and restrictions associated with the study
of anatomical specimens eg. use of gloves, toxic fumes, contagions
etc, and are more robust than the original specimen.
- Articles
and Abstracts - Some portions of my articles are given
below.
- Photo
Gallery - Some examples of plastinated specimens.
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Gary
goes cheerfully about his work
... plastinating piglets. |
A
Brief History - Gary Richard Whittaker(BAppSc)
- Gary
Whittaker born 1966 Wakefield England, arrived to Western
Australia 1967.
- Studied
at Curtin University of Technology, graduated in 1988 with
a BAppSc (Human Biology).
- In
1988/9 gained experience in prosection at Curtin's Anatomy
Dept. and 1989 employed by Curtin as Laboratory Technician
in the Anatomy facility.
- 1990
Tutored Human Biology including anatomy, histology and physiology.
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Conferences
Attended & Presentation:
1991
: 2nd Interim Conference on Plastination Los Angeles,
U.S.A.
1994
: 7th International Conference on Plastination. Graz,
Austria.
1995
: Australasian Society for Human Biology. Perth ,
Australia
1995
: Australian Optmetrical Association. Perth , Australia.
1996
: 8th International Conference on Plastination.
Brisbane, Australia.
1997
: 16th Annual Convention of the Philippine
Scoiety of Anatomists. Manila, Philippines.
1998
: 2nd National First Year Experience Conference.
Mandurah, Australia.
1999
: 6th Interim Conference on Plastination,
Rochester, New York.
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1993
- Curtin now has a fully operational plastination lab.
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1994
- Attended the 7th International conference on plastination
in Graz, Austria - To learn about new implementation of
the plastination technique.
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Other
qualifications; Instructor for Red Cross Society, Swimming
pool managers Certificate, a member of the International
Society for Plastination.
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Other
interests; swimming, squash, films.
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Articles
(Published)
Whittaker
G., Fyfe G. :Student Participation in Plastination as a Learning
Exercise in a Science Degree Embryology Unit. J Int Soc Plastination,
13 (1), 26-29, 1998.
Posters
Whittaker
G. "Anatomy without Tears" - Plastinated Specimens in Human Biology.
The Australasian Society for Human Biology Conference. 4-8th Dec.1994.
Perth Australia.
Oral Presentations
Whittaker
G. "Plastination in Teaching Anatomy" Australian Optometrical Association
Conference, 25-26th Dec. 1995 Perth, Australia
Whittaker
G., Fyfe G., "Student Participation in Plastination as a Learning
Exercise in a Science Degree Embryology Unit. " 8th International
Conference on Plastination, 14-19th July 1996 Brisbane,
Australia.
Whittaker
G. "Alternative Preservation Techniques for Anatomical Specimens."
16th Annual Convention of the Philippine Society of Anatomists.
Guest Speaker 13th Dec. 1997 Manila, Philippines.: "
Local
Radio 6NR - "Science at Curtin" - Plastination.
10min
intereview, April, 1998.
Whittaker
G. "Plastination- A Unique way to Preserve Anatomical Specimens."
Australian Red Cross Instructors Workshop. June 1998 Perth Australia.
Abstracts
Fyfe
G.M., Fyfe S.D., Donovan J.,Whittaker G., Strack R., Doornbusch
M.1998 Walking the tightrope of collaborative learning with first
year undergraduates. 2nd National First Year Experience Conference
Mandurah,1998, 21.
Whittaker
G., Fyfe G. 1995 Student Participation in Plastination as a Learning
Exercise in a Science Degree Embryology Unit. J Int Soc Plastination,
11 (1) 1996, 14.
Barton
J., Whittaker G., Jordan S. 1996 Trabeculae in the Nasal Cavity
of the Quokka(Sentonix brachyurus). 7th Annual Combined Biological
Sciences Meeting. Perth 1996, P14
"ANATOMY
WITHOUT TEARS ": PLASTINATED SPECIMENS IN HUMAN BIOLOGY
G.
R. WHITTAKER, Curtin University of Technology,Perth, Western Australia,
6001
Plastination
is a method used for the preservation of perishable biological specimens,
especially for soft, putrifiable organs with a high water content
eg. brain, heart, liver, muscle, joint specimens and body slices.
Plants, insects and animals can also be plastinated. Specimens preserved
in this manner are permanent, clean, non-toxic, dry and allow the
study of the anatomical function, textures and other properties
of the tissue which are lost with typical preservation techniques.
It also allows the handling and examination of the specimens without
the burden of gloves and toxic fumes eg formalin, phenol etc.
During
the plastination process the tissue water and lipid is replaced
by a curable polymer. The optical qualities (opaque or transparent)
as well as the mechanical properties (flexible or firm) depend on
the type of polymer used. Over the past three years a fully operational
plastination laboratory has been setup in the Department of Human
Biology at Curtin University and is currently producing plastinated
specimens which will be utilized in the undergraduate program. The
plastination procedure and equipment required will be outlined.
The
end product will be an anatomical specimen practically indistinguishable
from the original. The plastinated specimen is easy to handle, more
robust, chemical free and can be easily stored. Plastinated specimens
have a unique position as teaching aids having the durability of
anatomical models but exhibiting accurate anatomical structures
whilst showing the individuality and variation found in all biological
specimens. It must be pointed out that plastinated specimens cannot
fully replace "wet" specimen handling but complement it in an teaching
environment which demands good quality specimens of high durability
which are pleasant to handle.
PLASTINATION
- A Teaching Initiative to be Considered.
G.
R. WHITTAKER, Curtin University of Technology Perth, Western Australia.
1995 Unpublished.
Teaching
Human Biology, at any level, relies not only the expertise of a
tutor but also on the availability and use of good teaching aids.
Until recently these have been models and diagrams, either simple
or complex to demonstrate anatomical features. The use of anatomically
accurate models and specimens is critically important when teaching
to health professionals, especially those in the medical sciences.
Within these fields traditional teaching has meant the use of cadaver
material (wet biological specimens) which are preserved in formaldehyde
in a sealed jar or as a "wet specimen".
All
teaching aids have limitations and those used for the biological
sciences are without exception. Diagrams and charts can only show
structures in two dimensions. Students forever find it difficult
to visualise a three dimensional structure just from a diagram.
Diagrams do not always give a good indication of size, shape, colour,
weight or texture. Models, though a step up from charts and diagrams
are very expensive and not always anatomically correct, also they
may not show all related structures. As with diagrams, models tend
to be too colourful eg. Arteries - red, veins - blue, nerves - yellow.
This is good to differentiate structures but it is far from being
lifelike. Potted specimens allow the viewing of anatomical specimens
but do not allow handling. Wet specimens allow "hands on" learning
but students and tutors have to contend with noxious formaldehyde
fumes and wearing gloves.
Plastinated
specimens are a relatively new and unique teaching aid that have
the durability of anatomical models and exhibit accurate anatomical
structures whilst also showing the individuality and variation found
in all biological specimens. This is achieved because plastinated
specimens are "real specimens" in which the water content of the
biological tissue is replaced with a curable polymer. This means
any specimen with a water content can be plastinated including leather,
wood, plants, insects and animals. The type of polymer used during
the impregnation process determines the optical (transparent or
opaque) and the mechanical (flexible or firm) properties of the
specimen. Polymer types include silicone rubber, epoxy resin and
polyester.
The
specimens produced by plastination have many advantages over other
teaching aids they are odourless, dry to touch, non toxic, will
last indefinitely and most importantly they are life-like. The most
frequently used polymer is silicone rubber in the S10 technique.
This is viewed as the standard method as it is the most widely used,
it fulfils the need of most teaching schools, always produces satisfactory
results and is the easiest of all the techniques to implement. The
S10 method is currently in use at the Department of Human Biology
at Curtin University of Western Australia providing plastinated
specimens for undergraduate, as well as graduate courses.
The
four steps of the plastination process are outlined below.
- Fixation
or Tissue preparation - Requires the specimens to be fixed in
a 10% formaldehyde solution, this stablises the tissue and prevents
autolysis. Specimens can also be dissected and blood vessels injected
with a coloured medium to highlight desired structures.
- Dehydration
- All biological specimens have a high water content which needs
to be removed for plastination. This is achieved by a process
known as Freeze Substitution where the specimens are placed into
cold -25oC solvent, usually acetone. Over a period 4-5 weeks the
tissue water is slowly replaced by the acetone.
- Forced
impregnation - The dehydrated specimens are submerged into the
liquid polymer and placed under vacuum. The vacuum draws out the
acetone from the specimen and the polymer, takes its place.
- Gas
Cure - The polymer filled specimen is placed into a sealed chamber
where it comes in contact with a curing gas. This gas will harden
the polymer throughout the specimen, making the specimen dry to
touch with 48 hours. In a few months curing is complete and the
specimen can be stored indefinitely at room temperature.
The
end product is an anatomical specimen practically indistinguishable
from the original. The plastinated specimen is easy to handle, more
robust, chemical free and can be easily stored. Students tend to
find plastinated specimens more pleasant to handle than "wet specimens".
Plastinated specimens also have their limitations, they are relatively
inflexible and structures cannot be manipulated or easily reflected
to reveal underlying features. Dissection after plastination is
also limited but with careful tissue preparation before plastination
an extremely useful and some-what superior specimen can be produced.
Plastinated
specimens, prepared in many institutions worldwide, have been widely
accepted particularly due to their hard-wearing, durable nature
and their high teaching value. They complement existing teaching
initiatives in a variety of disciplines from general biology courses
through to specialised courses in anatomy, pathology, zoology and
forensic medicine, even research. Using these specimens as an adjunct
to other aids can only help the teaching of what is considered by
some a difficult and complex area.
For further
information
Gary Whittaker (Senior Technician)
Department of Human Biology
School of Biomedical Sciences
Curtin University of Technology
GPO box U 1987
Perth Western Australia 6001
Ph : (09) 351 7222 Fax : (09) 351 2339
E-mail : g.whittaker@curtin.edu.au |
The International Society for Plastination
Robbie Boyes (Far East Correspondent)
International Society for Plastination
University of Queenland
Royal Brisbane Hospital
Herston, Queensland, Australia 4029
Ph : (07) 253 8111 Fax : (07) 365 5539
E-mail : anrboyes@dingo.cc.uq.oz.au |
Abstract
of article presented at the Eighth International Conference of Plastination.
July 1996. Brisbane, Australia.
Student
Participation in Plastination as a Learning Exercise in a Science
Degree Embryology Unit.
G.Whittaker
and G.M.Fyfe
Department of Human Biology
School of Biomedical Sciences
Curtin University of Technology
P.O.Box U 1987, Perth WA 6001
Students
enrolled in their third year of a Bachelor of Science course at
Curtin University used plastination techniques to preserve their
dissected specimens as part of the practical component of the embryology
module of the Human Structure and Development unit co-ordinated
by GMF at Curtin University. The experience and expertise of GW
in S10 plastination technique was utilised. Students attended an
information session on the methodology of plastination, which related
well to their experience and knowledge of histological techniques
using resin embedding. They then examined foetal piglet specimens,
measured and weighed them to determine their various ages, and subsequently
decided which aspects of development they wished to portray in their
plastinated specimens.
Dissection
work was carried out by each student, and the resulting specimens
were dehydrated and plastinated over several weeks. Progress was
monitored by the students with respect to dehydration, shrinkage
and colour retention. After 8 weeks the plastinated specimens were
examined and their component parts identified. The specimens were
photographed with a digital camera and the resulting images imported
into a hypercard stack representing the work of the class. The images
were labelled and linked to information about their age and sectioning,
and the plastinated specimens retained for use in the next years
class.
As
a result of participation in the plastination process, students
were engaged in the technical aspects of their work and this
increased their feelings of responsibility to the resulting dissection.
Students gained insight into fixation and embedding techniques
combined with image digitisation and multimedia presentation
of graphical and text information.
Results
of student evaluation of the plastination component of the unit,
and examples of their work, will be presented with this paper.
Photo
Album
USEFUL
LINKS
Institute
for Plastination- Heidelberg
www.plastination.com/
Plastination
at Lausanne
www.ibcm.unil.ch/teach/plast
Plastination
Laboratory of Anatomy and Cell Biology at the University of Michigan
www.med.umich.edu/anatomy/plastinate/
Plastination
at Vienna University
www.univie.ac.at/anatomie2/plastination.html
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